Zabala, B.A., et al. Induction of Sustained Immunity Following Vaccination with Live Attenuated Trypanosoma cruzi Parasites Combined with Saponin-Based Adjuvants. Biology (Basel), 2025 Sep 20, 14(9):1298.

Zabala, B.A., et al. Induction of Sustained Immunity Following Vaccination with Live Attenuated Trypanosoma cruzi Parasites Combined with Saponin-Based Adjuvants. Biology (Basel), 2025 Sep 20, 14(9):1298. PMID: 41007442

• This study evaluates a vaccine strategy against Chagas disease using live attenuated Trypanosoma cruzi parasites (TCC strain) combined with saponin-based adjuvants (ISPA and Quil-A). 
• The findings demonstrate that while all formulations confer strong protection and reduce parasite load in mice, the inclusion of adjuvants enhances immune response regulation, promotes a balanced Th1/Th2 cytokine profile, and reduces tissue damage and inflammation, thereby improving safety. Specifically, adjuvants support sustained IL-10 production and foster long-term immune memory without parasite persistence, suggesting their potential to optimize vaccine efficacy and safety. 
• Although a human vaccine remains a future goal, this research provides promising insights for developing safer, more effective veterinary vaccines to control transmission and contribute to broader Chagas disease management strategies.

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