{"id":3638,"date":"2025-06-11T11:46:40","date_gmt":"2025-06-11T14:46:40","guid":{"rendered":"https:\/\/desertkingdev.wpenginepowered.com\/?p=3638"},"modified":"2025-06-11T11:52:26","modified_gmt":"2025-06-11T14:52:26","slug":"hu-j-et-al-herpes-simplex-virus-1-fusion-glycoprotein-b-h516p-prefusion-mutation-had-no-effect-on-vaccine-immunogenicity","status":"publish","type":"post","link":"https:\/\/desertking.com\/es\/hu-j-et-al-herpes-simplex-virus-1-fusion-glycoprotein-b-h516p-prefusion-mutation-had-no-effect-on-vaccine-immunogenicity\/","title":{"rendered":"Hu, J., et al. Herpes simplex virus 1 fusion glycoprotein B H516P prefusion mutation had no effect on vaccine immunogenicity."},"content":{"rendered":"<p><strong>Hu, J., et al. Herpes simplex virus 1 fusion glycoprotein B H516P prefusion mutation had no effect on vaccine immunogenicity. <\/strong><\/p>\n<p><strong><i>Vaccine, <\/i>2025 May 31, 57:127241. PMID: 40354699<\/strong><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">This study investigates the effect of the H516P mutation in HSV-1 glycoprotein B (gB), designed to stabilize the protein&#8217;s prefusion conformation, on vaccine immunogenicity. The\u00a0 immune responses of both mRNA and adjuvanted subunit vaccines containing either the wild-type or mutated gB were evaluated in mice.\u00a0\u00a0<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The main effect of QS-21, as reported in the study, is its ability to significantly enhance cellular immune responses. Specifically, QS-21 increased the production of IL-2 and IFN-\u03b3 by CD4+ and CD8+ T cells, as shown by flow cytometry analyses. Compared to alum-adjuvanted vaccines, the combination of QS-21 with CpG ODNs elicited higher levels of T-cell responses, including increased proportions of IL-2-producing T cells, up to 0.15% for CD4+ T cells and 0.13% for CD8+ T cells. This indicates that QS-21 boosts T-cell activation and cytokine production, which are essential for effective cellular immunity against HSV-1.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The mRNA vaccine elicited a cellular immune response level comparable to that induced by the subunit vaccine combined with QS-21 and CpG ODNs.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400;\">The results demonstrated that the H516P mutation did not significantly alter humoral or cellular immunity compared to the wild-type. Additionally, the study found that combining adjuvants QS-21 and CpG ODNs augmented cellular immune responses more effectively than aluminum. Importantly, all vaccinated mice were protected from infection with a virulent HSV-1 strain, highlighting the potential of these vaccine strategies despite the mutation&#8217;s limited impact on immunogenicity.<\/span><\/li>\n<\/ul>\n<p>Click\u00a0<a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/40354699\/\" target=\"_blank\" rel=\"noopener nofollow\">here<\/a>\u00a0to access the full scientific paper.<\/p>\n[arrow_forms id=\u00bb3298&#8243;]\n","protected":false},"excerpt":{"rendered":"<p>Hu, J., et al. Herpes simplex virus 1 fusion glycoprotein B H516P prefusion mutation had no effect on vaccine immunogenicity. Vaccine, 2025 May 31, 57:127241. PMID: 40354699 This study investigates the effect of the H516P mutation in HSV-1 glycoprotein B (gB), designed to stabilize the protein&#8217;s prefusion conformation, on vaccine immunogenicity. The\u00a0 immune responses of [&hellip;]<\/p>\n","protected":false},"author":16,"featured_media":3637,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"rs_blank_template":"","rs_page_bg_color":"","slide_template_v7":"","footnotes":""},"categories":[46],"tags":[],"class_list":["post-3638","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-texto-cientifico"],"_links":{"self":[{"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/posts\/3638","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/comments?post=3638"}],"version-history":[{"count":0,"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/posts\/3638\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/media\/3637"}],"wp:attachment":[{"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/media?parent=3638"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/categories?post=3638"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/desertking.com\/es\/wp-json\/wp\/v2\/tags?post=3638"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}